Part:BBa_K817031:Design

PfadBA-TetR(P0340)-pLac

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2157
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 3178
  

Design Notes

We add repressor TetR P0340 and repressor PLac I763027 sequentially in the back of K817002. The double repressor is designed to sharpen the promoter responsive curve.

Source

K817002 - P0340 - I763027